Photo of Stephen P.J. Brooks

Stephen P.J. Brooks

Adjunct Research Professor, Research Scientist, Nutrition Research Division

Degrees:B.Sc., MSc. (Brock), PhD. (Michigan State)
Office:Nutrition Research Division
Bureau of Nutritional Sciences
Food Directorate
Health Products and Food Branch
Sir Frederick Banting Research Centre
Ottawa, ON K1A 0L2
Website:Visit my lab website

Research Interests

Our research focuses on the effects of diet on the structure of the bacterial communities in the gastrointestinal tract and their relationship to physiological outcomes.  We use high-throughput sequencing of the V4 region as well as metagenomics analysis to follow changes in bacteria composition.  We follow immunological changes by flow cytometry and monitor gut permeability using GC and HPLC. We monitor gene expression changes in colonocytes using Next-Gen Sequencing.  We primarily use animal models to study the interaction of dietary factors and altered physiological states (diabetes, high fat diet-induced obesity, and colon cancer) on bacterial populations. Bioinformatics is an important part of the analysis chain.  We are trying to understand the importance of host-bacterial interactions in maintenance of health to sort out hype from reality.