{"id":3260,"date":"2013-09-20T11:45:04","date_gmt":"2013-09-20T15:45:04","guid":{"rendered":"http:\/\/carleton.ca\/biology\/?post_type=cu_event&#038;p=3260"},"modified":"2026-02-23T15:51:58","modified_gmt":"2026-02-23T20:51:58","slug":"adam-rudner","status":"publish","type":"cu_event","link":"https:\/\/carleton.ca\/biology\/event\/adam-rudner\/","title":{"rendered":"Adam Rudner"},"content":{"rendered":"<header class=\"mb-6 cu-pageheader cu-component-updated md:mb-12\">\n    <h1 class=\"cu-prose-first-last font-semibold !mt-2 mb-4 md:mb-6 text-3xl md:text-4xl lg:text-5xl lg:leading-[3.5rem] relative after:absolute after:h-px after:bottom-0 pb-5 after:w-10 after:bg-cu-red after:left-px\">\n        \n    <\/h1>\n    \n        <\/header>\n\n    \n    \n    \n    \n    <div class=\"cu-buttongroup cu-component-updated flex flex-wrap md:flex-1 gap-3 md:gap-5 justify-start\">\n                                                                        <\/div>\n    \n<h2 id=\"regulation-of-anaphase-onset\" class=\"wp-block-heading\">Regulation of Anaphase Onset<\/h2>\n\n\n\n<p>Adam Rudner, Ottawa Institute of Systems Biology, Univ. of Ottawa<\/p>\n\n\n\n<p>Faculty Host: Owen Rowland<\/p>\n\n\n\n<p>Cancer \u2022 Chromosomal disorders \u2022 Proteins \u2022 Yeast \u2022 Cellular Proteomics<\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<p>The exit from mitosis in eukaryotes is triggered by the regulated destruction of anaphase regulators by the Anaphase Promoting Complex (APC), a multi-subunit ubiquitin ligase. In yeast, the APC is composed of thirteen subunits and its activity is regulated by the binding of activating subunits, the presentation of its substrates, and by its phosphorylation. The cyclin dependent kinase, Cdk1, phosphorylates the APC and this phosphorylation is required for its activation during mitosis. In an effort to determine if phosphorylation regulates other aspects of APC function we are determining the complete phosphoproteome of the APC using mass spectrometry. To date we have identified many new phosphorylation sites on eight of the thirteen subunits. We are now using stable isotope labeling by amino acids in cell culture (SILAC) and absolute quantification (AQUA) to identify which sites are regulated during the cell cycle and in response to changes in cell physiology, as well to determine which kinases and phosphatases regulate APC phosphorylation.<\/p>\n\n\n\n<p>&nbsp;<\/p>\n","protected":false},"author":2,"featured_media":0,"template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"cu_event_type":[],"cu_event_audience":[],"class_list":["post-3260","cu_event","type-cu_event","status-publish","hentry"],"acf":{"cu_event_start_date":"2013-10-25T15:30:00","cu_event_end_date":"2013-10-25T16:30:00","cu_event_location_type":"in-person","cu_event_meeting_address_type":"on-campus","cu_building":"TT","cu_event_meeting_room":"4440Q","cu_event_meeting_address_full":null,"cu_event_virtual_type":"tbd","cu_event_virtual_meeting_link":"","cu_post_thumbnail":false,"cu_event_cost":"","cu_event_registration":"","cu_event_secondary_button":"","cu_event_contact_name":"Owen Rowland","cu_event_email":"owen.rowland@carleton.ca","cu_event_phone":""},"_links":{"self":[{"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/cu_event\/3260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/cu_event"}],"about":[{"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/types\/cu_event"}],"author":[{"embeddable":true,"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":0,"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/cu_event\/3260\/revisions"}],"wp:attachment":[{"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/media?parent=3260"}],"wp:term":[{"taxonomy":"cu_event_type","embeddable":true,"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/cu_event_type?post=3260"},{"taxonomy":"cu_event_audience","embeddable":true,"href":"https:\/\/carleton.ca\/biology\/wp-json\/wp\/v2\/cu_event_audience?post=3260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}