{"id":3358,"date":"2019-11-15T08:32:14","date_gmt":"2019-11-15T13:32:14","guid":{"rendered":"https:\/\/its-cuthemedev1.carleton.ca\/chemistry\/?post_type=cu_event&#038;p=3358"},"modified":"2025-08-15T11:56:33","modified_gmt":"2025-08-15T15:56:33","slug":"occi-seminar-series-professor-ann-english","status":"publish","type":"cu_event","link":"https:\/\/carleton.ca\/chemistry\/event\/occi-seminar-series-professor-ann-english\/","title":{"rendered":"OCCI Seminar Series: Professor Ann English"},"content":{"rendered":"<header class=\"mb-6 cu-pageheader cu-component-updated md:mb-12\">\n    <h1 class=\"cu-prose-first-last font-semibold !mt-2 mb-4 md:mb-6 text-3xl md:text-4xl lg:text-5xl lg:leading-[3.5rem] relative after:absolute after:h-px after:bottom-0 pb-5 after:w-10 after:bg-cu-red after:left-px\">\n        \n    <\/h1>\n    \n        <\/header>\n\n    \n    \n    \n    \n            <div class=\"cu-buttongroup cu-component-updated flex flex-wrap md:flex-1 gap-3 md:gap-5 justify-start\">\n                                                                                                        <\/div>\n        \n<h2 id=\"how-mass-spectrometry-empowers-the-study-of-metalloprotein-redox-reactions-in-the-test-tube-and-cell\" class=\"wp-block-heading\"><span class=\"fontstyle0\">How mass spectrometry empowers the study of metalloprotein redox reactions&nbsp; in the test tube and cell<\/span><\/h2>\n\n\n\n\n\n<p class=\"wp-block-paragraph\"><span class=\"fontstyle0\"><strong>Professor Ann English<\/strong><br>\nDepartment of Chemistry and Biochemistry<br>\n<\/span><span class=\"fontstyle0\">Concordia University<\/span><\/p>\n\n\n\n\n\n<p class=\"wp-block-paragraph\"><span class=\"fontstyle0\">Close to 40 years ago the Gray group at Caltech \u201cruthenated\u201d cytochrome c with Ru(NH<\/span><span class=\"fontstyle0\">3<\/span><span class=\"fontstyle0\">)<\/span><span class=\"fontstyle0\">5 <\/span><span class=\"fontstyle0\">to study electron transfer through the protein matrix. Preparation and characterization of this derivative took close to six years to complete. Since then, the development of protein mass spectrometry (MS) has enabled us to characterize ruthenated derivatives of cytochrome c peroxidase (Ccp1) and myoglobin in a day. Furthermore, using LC-MS\/MS, we have extensively profiled oxidative posttranslational modifications (PTMs) of Ccp1 induced by H<\/span><span class=\"fontstyle0\">2<\/span><span class=\"fontstyle0\">O<\/span><span class=\"fontstyle0\">2 <\/span><span class=\"fontstyle0\">in yeast cells. Mapping the residues oxidized reveals that hole hopping protects Ccp1\u2019s heme from irreversible oxidative damage but weakens the heme-protein interaction. This enables heme recruitment for maturation of apocatalase (Cta1), a better H<\/span><span class=\"fontstyle0\">2<\/span><span class=\"fontstyle0\">O<\/span><span class=\"fontstyle0\">2 <\/span><span class=\"fontstyle0\">scavenging enzyme than Ccp1. Such unprecedented heme mobilization is illustrative of the new redox reactions of metalloproteins emerging from their analyses by high-performance <\/span><span class=\"fontstyle2\">LC-MS\/MS<\/span><\/p>\n","protected":false},"author":2,"featured_media":0,"template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"cu_event_type":[28],"cu_event_audience":[],"class_list":["post-3358","cu_event","type-cu_event","status-publish","hentry","cu_event_type-event"],"acf":{"cu_event_start_date":"2019-11-15T10:00:00","cu_event_end_date":"2019-11-15T11:00:00","cu_event_add_to_calendar":"show","cu_event_location_type":"in-person","cu_event_meeting_address_type":"on-campus","cu_event_virtual_type":"tbd","cu_event_virtual_meeting_link":"","cu_building":"TB","cu_event_meeting_room":"447","cu_event_meeting_address_full":null,"cu_post_thumbnail":false,"cu_event_cost":"","cu_event_registration":"","cu_event_secondary_button":"","cu_event_contact_name":"","cu_event_email":"","cu_event_phone":""},"_links":{"self":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event\/3358","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event"}],"about":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/types\/cu_event"}],"author":[{"embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/users\/2"}],"version-history":[{"count":2,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event\/3358\/revisions"}],"predecessor-version":[{"id":3360,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event\/3358\/revisions\/3360"}],"wp:attachment":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/media?parent=3358"}],"wp:term":[{"taxonomy":"cu_event_type","embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event_type?post=3358"},{"taxonomy":"cu_event_audience","embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_event_audience?post=3358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}