{"id":3087,"date":"2019-03-18T09:18:45","date_gmt":"2019-03-18T13:18:45","guid":{"rendered":"https:\/\/its-cuthemedev1.carleton.ca\/chemistry\/?page_id=3087"},"modified":"2025-08-18T14:52:14","modified_gmt":"2025-08-18T18:52:14","slug":"advances-in-method-development-for-the-analysis-of-cyanobacterial-toxins-microcystins-and-anatoxin-a-in-the-great-lakes","status":"publish","type":"page","link":"https:\/\/carleton.ca\/chemistry\/occi-lecture-series\/advances-in-method-development-for-the-analysis-of-cyanobacterial-toxins-microcystins-and-anatoxin-a-in-the-great-lakes\/","title":{"rendered":"Advances in method development for the analysis of cyanobacterial toxins (microcystins and anatoxin-A) in the Great Lakes"},"content":{"rendered":"\n<section class=\"w-screen px-6 cu-section cu-section--white ml-offset-center md:px-8 lg:px-14\">\n    <div class=\"space-y-6 cu-max-w-child-5xl  md:space-y-10 cu-prose-first-last\">\n\n            <div class=\"cu-textmedia flex flex-col lg:flex-row mx-auto gap-6 md:gap-10 my-6 md:my-12 first:mt-0 max-w-5xl\">\n        <div class=\"justify-start cu-textmedia-content cu-prose-first-last\" style=\"flex: 0 0 100%;\">\n            <header class=\"font-light prose-xl cu-pageheader md:prose-2xl cu-component-updated cu-prose-first-last\">\n                                    <h1 class=\"cu-prose-first-last font-semibold !mt-2 mb-4 md:mb-6 relative after:absolute after:h-px after:bottom-0 after:bg-cu-red after:left-px text-3xl md:text-4xl lg:text-5xl lg:leading-[3.5rem] pb-5 after:w-10 text-cu-black-700 not-prose\">\n                        Advances in method development for the analysis of cyanobacterial toxins (microcystins and anatoxin-A) in the Great Lakes\n                    <\/h1>\n                \n                                \n                            <\/header>\n\n                    <\/div>\n\n            <\/div>\n\n    <\/div>\n<\/section>\n\n\n\n<section class=\"w-screen px-6 cu-section cu-section--white ml-offset-center md:px-8 lg:px-14\">\n    <div class=\"space-y-6 cu-max-w-child-5xl  md:space-y-10 cu-prose-first-last\">\n\n        \n    <div class=\"grid gap-6 mx-auto my-6 cu-column md:gap-10 md:grid-cols-2 md:my-10 first:mt-0\">\n        \n<div class=\"cu-column-content\">\n    \n\n<p><strong>Speaker<\/strong>: Dr. Xavier Ortiz Almirall, Development Scientist, Toxic Organic Section, Ontario Ministry of the Environment, Conservation and Parks.<br><strong>Title<\/strong>: Advances in method development for the analysis of cyanobacterial toxins(microcystins and anatoxin-A) in the Great Lakes<br><strong>Date, Time and Place<\/strong>: March 8, 2019, TB 202<\/p>\n\n\n<\/div>\n\n\n<div class=\"cu-column-content\">\n    \n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"160\" height=\"191\" src=\"https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall-160x191.jpg\" alt=\"Dr. Almirall photo\" class=\"wp-image-3088\" srcset=\"https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall-160x191.jpg 160w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall-240x286.jpg 240w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall-400x477.jpg 400w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall-360x429.jpg 360w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/almirall.jpg 421w\" sizes=\"auto, (max-width: 160px) 100vw, 160px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n<\/div>\n\n\n    <\/div>\n\n\n    <\/div>\n<\/section>\n\n\n\n<p>Due to Great Lakes eutrophication and raise of global temperatures caused by the Climate Change, occurrence of cyanobacteria harmful algal blooms have been increasing exponentially during the last decade in Ontario. These algal blooms can be severely toxic due to the production of cyanotoxins such as microcystins and anatoxin-A. There are several challenges associated with the analysis of these toxins. During this presentation, a selection of the Ontario Ministry of the Environment, Conservation and Parks\u2019 (MECP) research projects that address some of these problems will be discussed:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Sample preparation automation: Typical sample preparation for cyanotoxins analysis can take up to several days. An automated method for the analysis of microcystins and anatoxin-A using on-line solid phase extraction coupled to liquid chromatography will be showcased.<\/li>\n\n\n\n<li>Non-targeted analysis of microcystins: There are over 200 different microcystin variants reported in literature to date. A workflow for the non-targeted analysis of previously unknown variants using quadrupole-time of flight mass spectrometry (QTOF MS) will be presented (Fig. 1). Different advanced acquisition modes will be compared.<\/li>\n\n\n\n<li>Analysis of microcystins in fish: Microcystins in fish can get covalently bound to protein phosphatases, thus difficulting their analysis. Different analytical approaches to address this problem during sample preparation will be discussed.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"779\" height=\"434\" src=\"https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1.jpg\" alt=\"Microcysin\" class=\"wp-image-3090\" srcset=\"https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1.jpg 779w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1-160x89.jpg 160w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1-240x134.jpg 240w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1-768x428.jpg 768w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1-400x223.jpg 400w, https:\/\/carleton.ca\/chemistry\/wp-content\/uploads\/sites\/138\/microcystin-1-360x201.jpg 360w\" sizes=\"auto, (max-width: 779px) 100vw, 779px\" \/><\/figure>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Due to Great Lakes eutrophication and raise of global temperatures caused by the Climate Change, occurrence of cyanobacteria harmful algal blooms have been increasing exponentially during the last decade in Ontario. These algal blooms can be severely toxic due to the production of cyanotoxins such as microcystins and anatoxin-A. There are several challenges associated with [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":1779,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_cu_dining_location_slug":"","footnotes":"","_links_to":"","_links_to_target":""},"cu_page_type":[],"class_list":["post-3087","page","type-page","status-publish","hentry"],"acf":{"cu_post_thumbnail":""},"_links":{"self":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/pages\/3087","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/comments?post=3087"}],"version-history":[{"count":4,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/pages\/3087\/revisions"}],"predecessor-version":[{"id":5310,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/pages\/3087\/revisions\/5310"}],"up":[{"embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/pages\/1779"}],"wp:attachment":[{"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/media?parent=3087"}],"wp:term":[{"taxonomy":"cu_page_type","embeddable":true,"href":"https:\/\/carleton.ca\/chemistry\/wp-json\/wp\/v2\/cu_page_type?post=3087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}