Johann Schernthaner

Adjunct Professor, Research Scientist

Office:Eastern Cereal and Oilseed Research Centre
Agriculture and AgriFood Canada
960 CARLING AVE, Bldg. 21,
Central Experimental Farm
Ottawa, ON K1A 0C6

Areas of Expertise

  • containment of transgenes in plants
  • storage protein genes
  • full-length cdna libraries
  • high throughput promoter libraries
  • genomic coding and flanking regions
  • edible vaccines in plants
  • molecular genetics
  • seed development
  • abiotic stress

Current Research

We are currently studying the biochemical factors that contribute to latex and rubber quality/quantity in Taraxacum kok-saghyz (Russian dandelion). This plant is currently considered to be a potential industrial crop for the production of natural rubber (polyisoprene) and latex rubber. We are generating proteome maps of latex-associated proteins to do comparative studies between the rubber producing Russian dandelion and the common dandelion, Taraxacum officinale. The proteome maps are complemented by RNA profiling with RNA sequences obtained from massive parallel sequencing (MPS).

Selected Publications

Schernthaner, J.P., Fabijanski, S.F., Arnison, P.G., Racicot, M., and Robert, L.S. (2003). “Control of seed germination in transgenic plants based on the segregation of a two-component genetic system.”, Proceedings of the National Academy of Sciences of the USA (PNAS), 100(11), pp. 6855-6859.

Piché, C. and Schernthaner, J.P. (2005). “Optimization of in Vitro Transcription and Full-Length cDNA Synthesis Using the T4 Bacteriophage Gene 32 Protein.”, Journal of Biomolecular Techniques, 16(3), pp. 239-247.

Singh, J., Lacroix, G., Douglas, J., Dubuc, E., Allard, G.C., Keller, W., and Schernthaner, J.P. (2009). “The use of Group 3 LEA proteins as fusion partners in facilitating recombinant expression of recalcitrant proteins in E. coli.”, Protein Expression and Purification 67; 15-22.

Schernthaner, J.P., Singh, J., Labbé, N., Liao, D., and Marsolais, F. (2011). “The unfolded protein response in plant seeds.”, in Hendricks, B.P. (ed.) – Agricultural Research Updates. Vol. 2, Nova Science Publishers Inc.

Zifkin M, Jin A, Ozga JA, Zaharia LI, Schernthaner JP, Gesell A, Abrams SR, Kennedy JA, Constabel CP.(2011).Gene expression and metabolite profiling of developing highbush blueberry fruit indicates transcriptional regulation of flavonoid metabolism and activation of abscisic Acid metabolism. Plant Physiol. 2012 Jan;158(1):200-24. Epub 2011 Nov 15.